• Blog Posts

  • How do you read a strand of DNA?

    To carry on with the theme of DNA, I thought today we would talk about a process downstream from PCR – Sequencing! Sequencing is the process of determining the order of the four base pairs in a DNA strand. There are three primary methods of DNA sequencing: Sanger sequencing, Next-generation sequencing, and third generation sequencing methods. Sanger sequencing was the first major [...]

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    Polymerase Chain Reaction (PCR) at TSoG

    Polymerase Chain Reaction (PCR) is the technique used by biochemists and molecular biologists to amplify one piece of DNA and generate millions of copies. At TSoG we have utilized PCR on several occasions. As part of SEADD 2017 we amplified the 16s rRNA gene of soil bacteria isolated from the Girl Scouts Camp Whispering Cedars. The 16s rRNA gene is the DNA sequence that encodes part of the small [...]

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    Mastering the Art of the Stupid Question

    One of the things that I am most thankful for is that I am surrounded by friends and family who are willing to participate in nearly continuous conversations about our students, our programs, and our curriculum. My wife is a part of at least half of these discussions, and, despite her lack of technical expertise, cares just as deeply about the outcomes of our students as I do. Rather than [...]

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